Antimicrobial and Cytotoxic Potential of Anemone tetrasepala Royle

Abstract

Anemone tetrasepala Royle is a medicinal herb commonly used in folk medicine to treat various diseases. In this study, we aimed to evaluate the antimicrobial and cytotoxic potential of the methanolic extract of Anemone tetrasepala Royle, a wild plant grown in Dunga Gali, district Abbottabad, against Brine Shrimp larvae and bacterial strains. Our results showed that the methanolic extract of the plant exhibited significant cytotoxic potential at concentrations of 10ppm, 100ppm, and 1000ppm. Notably, the extract had the most potent cytotoxic effect on the Brine Shrimp larvae, with significant mortality observed at 100ppm and 1000ppm of the leaves extract (91% and 100%), as compared to the root extract. Furthermore, the methanolic extract of Anemone tetrasepala Royle also exhibited significant antimicrobial activity against the tested bacterial strains. These findings suggest that Anemone tetrasepala Royle has the potential as a natural source of cytotoxic and antimicrobial agents.