GC-MS analysis, antimicrobial, antioxidant, phytochemical, and phytotoxic activities of Elaeagnus angustifolia L. using different extracts

Authors

  • Aiman Laraib Syed Department of Botany, Hazara University, Mansehra, Pakistan
  • Ghulam Mujtaba Shah Department of Botany, Hazara University, Mansehra, Pakistan
  • Saleha Ashfaq Department of Biology, University of Haripur, Haripur, Khyber Pakhtunkhwa, Pakistan
  • Hafsa Abrar Department of Botany, Government Post-Graduate College No.1 Abbottabad, Pakistan.
  • Noor e Baseerat Department of Biology, University of Haripur, Haripur, Khyber Pakhtunkhwa, Pakistan
  • Naqash Khan University of haripur
  • Muhammad Hamza Department of Botany, Government Degree Collage Lahor, Swabi, Kp, Pakistan
  • Muhammad Abbas Hasan Department of Zoology, Islamia College University Peshawar, Khyber Pakhtunkhwa, 25120, Pakistan
  • Kareem Ullah Department of Biology, University of Haripur, Haripur, Pakistan
  • Kareem Ullah Department of Biology, University of Haripur, Haripur, Pakistan

Abstract

This study aimed to assess the phytotoxic, phytochemical, antioxidant, and antibacterial properties of Elaeagnus angustifolia, a therapeutic plant. As solvents, distilled water, methanol, and ethanol were used to make the plant extracts. Using the DPPH free radical scavenging activity, the antioxidant activity was ascertained, and the IC50 was calculated. The ethanolic extract of Elaeagnus angustifolia had 0.90±0.16 total flavonoid components, whereas the methanolic and aqueous extracts were found to contain 0.78±0.16 total phenolic contents. At 1.5 mg/ml, the antioxidant findings of Elaeagnus angustifolia's methanolic extract showed 0.78±0.18 percent inhibition and SCV 49.10%, while the ethanolic extract showed 0.54±0.12 percent inhibition with 64.28% SCV. The best-result-oriented Elaeagnus angustifolia extract underwent phytochemical analysis using the Gas Chromatography-Mass Spectrometry (GC-MS) method. The findings showed that a variety of compounds were present, with the most common ones being acetone (1.18%), ethyl acetate (38.95%), isobutyl acetate (2.71%), Phytol (3.57%), isoquinoline,1-[(3,4-diethoxyphenyl)methyl].n-Hexadecanoic acid (5.61%), 9-Octadecenoic acid, 1,2,3-propanetriyl ester (10.72%), Bis(2-ethylhexyl) phthalate (3.48%), Squalene (1.40%), Octadecanoic acid (1.78%), n-propyl acetate (4.09%),  2,2-Dimethyl-3-(3,7,16,20-tetramethyl (1.12%) and 1,6,10,14,18,22- Tetracosahexaen-3-ol (1.195%). In order to determine the phytotoxic potential of Elaeagnus angustifolia leaf and stem extracts, a growth bioassay was performed against Lemna minor. The current study demonstrated that in order to create efficient management strategies that drastically lessen the influence of the infections on both human health and the environment.

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Published

2023-12-30

How to Cite

Aiman Laraib Syed, Ghulam Mujtaba Shah, Saleha Ashfaq, Hafsa Abrar, Noor e Baseerat, Khan, N., Muhammad Hamza, Abbas Hasan, M. ., Ullah, K. ., & Ullah, K. . (2023). GC-MS analysis, antimicrobial, antioxidant, phytochemical, and phytotoxic activities of Elaeagnus angustifolia L. using different extracts. Phytopharmacology Research Journal, 2(3), 1–12. Retrieved from http://ojs.prjn.org/index.php/prjn/article/view/49

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