Pharmaceutical and Biomedical Characterization of Cannabinoids in Cannabis sativa L. using Gas Chromatography-Mass Spectrometry (GC-MS)

Abstract

Cannabis is the most frequently used drug of abuse not only in Pakistan as well as the rest of the globe. Its use is increasing drastically every year. Every year, its use rises dramatically. Cannabis sativa can be analyzed using GC-MS to reveal the variations in the components of this plant harvested from Tirah valley. Cannabidiol (CBD), one of the biologically active substances found in naturally occurring cannabinoids, was found in significant concentrations in both ethanolic and n-hexane extracts in addition to other cannabinoids. Understanding the plant's components will help determine its prevalence. When comparing the components of this drug of abuse, GC-MS is a helpful technique that can help the investigator determine the plant's origin. Comparison of both extracts also aids in the understanding and acquaintance of similarities of different cannabinoids. The main objective of the study was to investigate medicinally active compounds of C. sativa. Results from the study proved that Cannabis is not meant only for its recreational purposes but the presence of these medicinal compounds in high concentrations make it a valuable source to be used in herbal medicine for different ailments. Key words: Cannabis sativa, Cannabinoids, GC-MS