GC-MS analysis, antimicrobial, antioxidant, phytochemical, and phytotoxic activities of Elaeagnus angustifolia L. using different extracts
Abstract
This study aimed to assess the phytotoxic, phytochemical, antioxidant, and antibacterial properties of Elaeagnus angustifolia, a therapeutic plant. As solvents, distilled water, methanol, and ethanol were used to make the plant extracts. Using the DPPH free radical scavenging activity, the antioxidant activity was ascertained, and the IC50 was calculated. The ethanolic extract of Elaeagnus angustifolia had 0.90±0.16 total flavonoid components, whereas the methanolic and aqueous extracts were found to contain 0.78±0.16 total phenolic contents. At 1.5 mg/ml, the antioxidant findings of Elaeagnus angustifolia's methanolic extract showed 0.78±0.18 percent inhibition and SCV 49.10%, while the ethanolic extract showed 0.54±0.12 percent inhibition with 64.28% SCV. The best-result-oriented Elaeagnus angustifolia extract underwent phytochemical analysis using the Gas Chromatography-Mass Spectrometry (GC-MS) method. The findings showed that a variety of compounds were present, with the most common ones being acetone (1.18%), ethyl acetate (38.95%), isobutyl acetate (2.71%), Phytol (3.57%), isoquinoline,1-[(3,4-diethoxyphenyl)methyl].n-Hexadecanoic acid (5.61%), 9-Octadecenoic acid, 1,2,3-propanetriyl ester (10.72%), Bis(2-ethylhexyl) phthalate (3.48%), Squalene (1.40%), Octadecanoic acid (1.78%), n-propyl acetate (4.09%), 2,2-Dimethyl-3-(3,7,16,20-tetramethyl (1.12%) and 1,6,10,14,18,22- Tetracosahexaen-3-ol (1.195%). In order to determine the phytotoxic potential of Elaeagnus angustifolia leaf and stem extracts, a growth bioassay was performed against Lemna minor. The current study demonstrated that in order to create efficient management strategies that drastically lessen the influence of the infections on both human health and the environment.
